How to use atomic absorption spectrophotometer _Atomic absorption spectrophotometer

Introduction to atomic absorption spectrophotometer

Atomic absorption spectrometer, also known as atomic absorption spectrophotometer, performs metal element analysis based on the effect of the atomic vapor of the ground state on the characteristic radiation absorption. It is capable of sensitively and reliably measuring trace or trace elements.

Atomic absorption spectrophotometer workflow

Taking the content of magnesium ions in the test solution as an example, the instrument device is shown in Figure 3-14. The test solution is sprayed into a mist and introduced into the flame. The droplets containing magnesium salt are evaporated at the flame temperature. Dissociation into magnesium atoms forms atomic vapor. When a hollow cathode lamp of magnesium is used as a light source, it emits a characteristic spectrum (wave) of magnesium having a wavelength of 285.2 nm. When it passes through a certain thickness of magnesium atomic vapor in the flame, part of the light is taken by the ground state magnesium atom in the vapor. Absorption increases the strength. After being separated by a monochromator, it is accepted by the detector, and the detector measures the degree of attenuation of the 285.2 nm line light of magnesium, and then the content of magnesium in the sample can be obtained.

Basic operation of atomic absorption spectrophotometer

1. Turn on the regulated power supply, and turn on the power switch after the voltage is stable at 220 volts;

2. Open the air press;

3. Open the main valve of the gas cylinder, and open the main valve of the acetylene cylinder at most one turn;

4. Open the exhaust fan and cooling water (when using graphite furnace atomization).

Second, the test

1. Install the hollow cathode lamp of the element to be tested, and adjust the lamp current and wavelength to the required value;

2. Ignition, set the instrument test parameters;

3. Insert the atomizer capillary into the deionized water, after zeroing, insert the nebulizer capillary into the solution until the absorbance

After the display is stable, record the test results, insert the nebulizer capillary into the deionized water, and wait until the instrument display returns to zero;

The other solutions were sequentially determined by the same method.

Third, shut down

1. After the test is completed, clean the atomization system by aspirating clean deionized water for several minutes;

2. Close the main valve of the gas cylinder, and close the gas valve of the instrument after the residual gas in the pipeline is burned;

3. Loosen the gas and gas-assisting knob on the instrument panel to rotate the lamp current to zero.

4. Turn off the instrument power supply, turn off the regulated power supply;

5. Close the fan and cooling water;

6. Loosen the gas cylinder pressure reducing valve;

7. Close the gas, air compressor, cathode lamp. Knob reset, turn off the photomultiplier tube negative high voltage power supply, total power supply;

8. Wipe the burning head with filter paper and fill in the instrument usage record;

9. Clean the glass instrument and reset it.

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